Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus

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Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus

BACKGROUND The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell factory. Due to the diversity of chemical and cell properties, an experimental protocol validated on B. methanolicus is needed. Here a systemati...

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Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus.

Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50 degrees C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph. Here we describe the DNA sequence and characterization of a 19,167-bp circular plasmid, designated pBM19, iso...

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a study on the effectiveness of textual modification on the improvement of iranian upper-intermediate efl learners’ reading comprehension

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The generic position of 14 strains of gram-positive bacteria able to use methanol as a growth substrate was determined. All are obligately aerobic, thermotolerant organisms that are able to grow at temperatures of 35 to 60 degrees C. Nine of the strains produce oval spores at a subterminal-to-central position in slightly swollen rod-shaped cells. DNA-DNA hybridization studies, 5S rRNA sequence ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1996

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.62.3.1107-1111.1996